Matching the immersion media to the sample is critical for microscopy. Water dipping allow immersion in media (upright systems) Oil has an RI (1. Match sample to objective Use correct immersion oil Use correct cover slip, plates etc optical grade Transmitted light dont forget, not all about fluorescence overlay fluorescence and transmitted light image to generate amazing structural context.Īchromat: limited color correction Fluorite: good all around objectives, NA up to 1.Īpochromat: Highly color corrected Plan Apochromat: corrected for field curvature.įor dim samples, youll want high NA, regardless of resolution, to maximize light-gathering.ĭry used in air Immersion drop of liquid between sample and lens front surface. Wash hands minimise oilscrud from fingers getting onto the lens surface.Ĭonfocal vs widefield which to use confocal microscopes are still constrained by the same diffraction limit of light as any other conventional (i. Spinning Disk images are very well suited for Huygens deconvolution with the Spinning Disk optical option, specially designed to take into account the optical properties of spinning disk microscopes. Spinning Disk Confocal Psf Series Of Equallyįor fixed samples, a point-scanning Confocal Microscope is, in some cases, a better solution.Ī Yokogawa Disk is a Nipkow disk with an array of microlenses in (or right after) the holes used in many commercial spinning disk microscopes. Spinning Disk Confocal Psf Series Of Equally.
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